Simplified Fluorescence Microscopy of Tubercle Bacilli
نویسنده
چکیده
The fluorescence of tubercle bacilli stained with auramine and rhodamine does not require ultra-violet light. It can be caused by blue light up to 406mp. A’copper sdphate ammonia liquid filter, suitably diluted, will transmit this waveband. A gelatin screen filter, absorbing all light below 510mp. is used in the eyepiece. Normal, high intensity projection Alament lamps, combined with lamp condensers of large aperture, provide suitable light sources. The numerical aperture of the microsoope condenser must be fully used by immersion in glycerol. The fluorescence is vellry bright with the usual biological, and certain binocular, microscopes. The simplicity of the equipment enables fluorescence microscopy of tubercle bacilli to be used at low cost in any laboratory.
منابع مشابه
Automated identification of tubercle bacilli in sputum. A preliminary investigation.
OBJECTIVE To use an automated method to detect tubercle bacilli in sputum specimens. STUDY DESIGN Using fluorescence microscopy, tubercle bacilli were identified on auramine-stained sputum specimens. Images were then captured with a digital camera and enhanced through imaging processing techniques. The bacilli were recognized using neural network classifiers. RESULTS This preliminary invest...
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تاریخ انتشار 2008